ARHGEF2 antibody (AA 21-120)
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- Target See all ARHGEF2 Antibodies
- ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))
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Binding Specificity
- AA 21-120
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ARHGEF2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Cow,Horse,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human GEF H1
- Isotype
- IgG
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))
- Alternative Name
- GEF H1 (ARHGEF2 Products)
- Synonyms
- MGC82333 antibody, MGC86214 antibody, MGC75855 antibody, AA408978 antibody, GEF antibody, GEF-H1 antibody, GEFH1 antibody, LFP40 antibody, Lbcl1 antibody, Lfc antibody, P40 antibody, mKIAA0651 antibody, GEF-H1/LFC antibody, Rho/Rac guanine nucleotide exchange factor 2 antibody, Rho/Rac guanine nucleotide exchange factor 2 S homeolog antibody, rho/rac guanine nucleotide exchange factor (GEF) 2 antibody, ARHGEF2 antibody, arhgef2.S antibody, arhgef2 antibody, Arhgef2 antibody
- Background
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Synonyms: GEF, P40, GEFH1, LFP40, GEF-H1, Rho guanine nucleotide exchange factor 2, Guanine nucleotide exchange factor H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, ARHGEF2, KIAA0651
Background: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans, acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2.
- Gene ID
- 9181
- UniProt
- Q92974
- Pathways
- Negative Regulation of intrinsic apoptotic Signaling
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