Perlecan/Heparan Sulfate Proteoglycan 2 antibody (Biotin)

Antigen: Perlecan/Heparan Sulfate Proteoglycan 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN709258

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Heparan Sulfate Proteoglycan 2

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: Basement membrane specific heparan sulfate proteoglycan core protein, Endorepellin (domain V region), Heparan Sulfate Proteoglycan, Heparan sulfate proteoglycan of basement membrane, HSPG 2, HSPG, Hspg2, LG3 peptide, Perlecan, PLC antibody Schwartz Jampel syndrome 1 (chondrodystrophic myotonia), SJA antibody SJS antibody SJS1 antibody.

Molecular Weight: 469kDa

Comments:

This gene encodes the perlecan protein

which consists of a core protein to which three long chains of glycosaminoglycans(heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin

prolargin

collagen type IV

FGFBP1

FBLN2

FGF7 and Transthyretin

etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix

where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g.

FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes

where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1

Silverman-Handmaker type of dyssegmental dysplasia

and Tardive dyskinesia.[provided by RefSeq

Mar 2010].

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only