NFKBIE antibody (pSer157) (FITC)

Catalog No. ABIN710733

Product Details anti-NFKBIE Antibody (FITC)

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Binding Specificity: pSer157

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIE antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human NFKBIE around the phosphorylation site of Ser157

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NFKBIE

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Alternative Name: Ikb epsilon (NFKBIE Products)

Synonyms: NFKBIE antibody, IKBE antibody, Slc35b2 antibody, NFKB inhibitor epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon antibody, NFKBIE antibody, Nfkbie antibody

Background:

Synonyms: NFKBIE phospho S157, IKB epsilon Ser157, IKB epsilon S, p-IKB epsilon Ser157, p-IKB epsilon S157, I kappa B epsilon, IkappaBepsilon, IkB E, IKBE, IKB epsilon, MGC72568, NF kappa B inhibitor epsilon, NF kappa BIE, NFkappa BIE, NFkappaB inhibitor epsilon, NFKBIE, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon, OTTHUMP00000016522, Slc35b2, solute carrier family 35, member B2.

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.

Gene ID: 4794

Pathways: NF-kappaB Signaling, Maintenance of Protein Location