Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RAF1 antibody (pTyr341)

RAF1 Reactivity: Human WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN712706
  • Target See all RAF1 Antibodies
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Binding Specificity
    • 49
    • 43
    • 35
    • 34
    • 32
    • 26
    • 24
    • 23
    • 23
    • 22
    • 19
    • 18
    • 15
    • 13
    • 13
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr341
    Reactivity
    • 447
    • 231
    • 159
    • 14
    • 13
    • 12
    • 12
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 478
    • 15
    • 2
    • 2
    Rabbit
    Clonality
    • 453
    • 44
    Polyclonal
    Conjugate
    • 202
    • 37
    • 28
    • 27
    • 20
    • 20
    • 20
    • 20
    • 20
    • 20
    • 17
    • 16
    • 10
    • 10
    • 10
    • 10
    • 10
    This RAF1 antibody is un-conjugated
    Application
    • 297
    • 216
    • 104
    • 103
    • 89
    • 75
    • 48
    • 31
    • 30
    • 23
    • 16
    • 11
    • 10
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Human
    Predicted Reactivity
    Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Tyr341
    Isotype
    IgG
    Top Product
    Discover our top product RAF1 Primary Antibody
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Wu, Dong, Shi, Yu, Fu, Zhang, Cai, Zhao, Peng, Wu, Wu: "Regulation of integrin ?V subunit expression by sulfatide in hepatocellular carcinoma cells." in: Journal of lipid research, Vol. 54, Issue 4, pp. 936-52, (2013) (PubMed).

  • Target
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Alternative Name
    c-Raf (RAF1 Products)
    Synonyms
    CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody
    Background

    Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF

    Background: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

    Gene ID
    5894
    UniProt
    P04049
    Pathways
    MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
You are here:
Support