FADD antibody (pSer194)
-
- Target See all FADD Antibodies
- FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
-
Binding Specificity
- pSer194
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This FADD antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human FADD around the phosphorylation site of Ser194
- Isotype
- IgG
- Top Product
- Discover our top product FADD Primary Antibody
-
-
- Application Notes
-
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
-
- Target
- FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))
- Alternative Name
- FADD (FADD Products)
- Synonyms
- MORT1 antibody, Mort1/FADD antibody, Mort1 antibody, TNFRSF6 antibody, adaptor antibody, fadd antibody, mort1 antibody, xfadd antibody, Fas associated via death domain antibody, Fas (TNFRSF6)-associated via death domain antibody, Fas associated via death domain S homeolog antibody, Fas (tnfrsf6)-associated via death domain antibody, FADD antibody, Fadd antibody, fadd.S antibody, fadd antibody
- Background
-
Synonyms: P-Fas-associated protein with death domain, FADD protein, Fas TNFRSF6 associated via death domain, Fas associated via death domain, Fas associating death domain containing protein, Fas associating protein, Fas associating protein with death domain, Fas TNFRSF6 associated via death domain, GIG 3, GIG3, Growth inhibiting gene 3 protein, H sapiens mRNA for mediator of receptor induced toxicity, Mediator of receptor induced toxicity , MGC8528 , MORT 1, MORT1, FADD_HUMAN.
Background: FADD (Fas Associated Death Domain) is an apoptosis adapter molecule enabling transduction of the apoptosis signal initiated via the FasL/Fas receptor interaction. The protein contains a C terminal death domain that interacts with the Fas receptor death domain. The N terminus contains a death effectors domain (DED) which recruits caspase to the death inducing signaling complex (DISC) and initiates the apoptotic caspase cascade. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through self-cleavage. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. FADD is implicated in non-apoptotic cellular pathways such as the regulation of cell cycle machinery in T lymphocytes. This is connected to the phosphorylation state of FADD and to the FasL/TRAIL induced transcriptional activation of cfos protooncogene. FADD also interacts with the hepatitis C virus core protein in the HEK 293 cell line.
- Gene ID
- 8772
- Pathways
- Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
-