CDH6 antibody (K-Cadherin) (AA 310-360)

Details for Product anti-CDH6 Antibody No. ABIN715286, Supplier: Log in to see
Antigen
  • KCAD
  • K-cadherin
  • cad6
  • cadherin-6
  • CAD6
  • c-cad6B
  • cadherin 6
  • cadherin 6, type 2, K-cadherin (fetal kidney)
  • Cdh6
  • CDH6
Alternatives
anti-Human CDH6 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 310-360
21
20
19
15
8
5
4
4
3
3
3
3
2
1
1
Reactivity
Human
137
28
10
1
1
Host
Rabbit
79
55
5
Clonality
Polyclonal
Conjugate
This CDH6 antibody is un-conjugated
8
5
5
5
5
5
4
4
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
88
62
46
44
29
25
20
16
12
4
2
2
2
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Lot Number 130823
Method validated Flow Cytometry
Positive Control 786-O cells and [MCF-7 cells] (http://www.nature.com/bjc/journal/v90/n5/fig_tab/6601640f10.html#figure-title)
Notes As expected, strong signal was observed in the cells stained with anti-K-Cadherin (CDH6) antibody. A small amount of non-specific staining was observed from the isotype and secondary antibody only negative controls compared with unstained cells.
Primary Antibody
  • Antigen: K-Cadherin (CDH6) antibody
  • Catalog number: ABIN715286
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130823
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive controls: 786-O cells (human renal cell carcinoma) and MCF-7 cells (human breast cancer)
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-Alexa 647 to confirm no background signal produced from secondary antibody alone
Protocol
  • Positive control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Propidium Iodide (PI) was added to discern live cells from dead cells.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Experimental Notes - The data displayed is gated on PI negative cells.
Validation Images
Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 310-360) antibody (ABIN715286) Figure 1. Histogram of 786-O cells stained with anti-K-cadherin (orange), isotype con...
Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 310-360) antibody (ABIN715286) Figure 2. Histogram of MCF-7 cells stained with anti-K-cadherin (orange), isotype con...
Immunogen KLH conjugated synthetic peptide derived from human K Cadherin
Isotype IgG
Purification Purified by Protein A.
Alternative Name K Cadherin (CDH6 Antibody Abstract)
Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Subcellular location: Extracellular

Synonyms: CAD6, KCAD, Cadherin-6, Kidney cadherin, K-cadherin, CDH6

Gene ID 1004
UniProt P55285
Pathways Cell-Cell Junction Organization
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-K-Cadherin (CDH6) (AA 310-360) antibody (ABIN715286) Formalin-fixed and paraffin embedded rat brain Rabbit Anti K Cadherin Polyclonal Anti...
Immunohistochemistry (IHC) image for anti-K-Cadherin (CDH6) (AA 310-360) antibody (ABIN715286) Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti K Cadherin P...
Flow Cytometry (FACS) image for anti-K-Cadherin (CDH6) (AA 310-360) antibody (ABIN715286) Images provided by the Independent Validation Program badge number 29743.Histogram of...
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