Vinculin antibody (AA 1001-1134)

Catalog No. ABIN719261

Product Details anti-Vinculin Antibody

Target: Vinculin (VCL)

Binding Specificity: AA 1001-1134

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Vinculin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Vinculin antibody (ABIN719261)

Yunes-Medina, Paciorkowski, Nuzbrokh, Johnson: "Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability." in: Molecular and cellular neurosciences, Vol. 86, pp. 72-80, (2018) (PubMed).

Dhawan, Pan, Shie, Chu, Huang, Chen, Chen: "The Spatiotemporal Control of Osteoblast Cell Growth, Behavior, and Function Dictated by Nanostructured Stainless Steel Artificial Microenvironments." in: Nanoscale research letters, Vol. 12, Issue 1, pp. 86, (2017) (PubMed).

Jiang, Yang, Li, Wu, Meng, Li: "Aqueous extracts of Tribulus terrestris protects against oxidized low-density lipoprotein-induced endothelial dysfunction." in: Chinese journal of integrative medicine, Vol. 22, Issue 3, pp. 193-200, (2016) (PubMed).

Dhawan, Pan, Lee, Chu, Huang, Lin, Chen: "Spatial Control of Cell-Nanosurface Interactions by Tantalum Oxide Nanodots for Improved Implant Geometry." in: PLoS ONE, Vol. 11, Issue 6, pp. e0158425, (2016) (PubMed).

He, Meng, Yao, Jiang, Wu, Wu: "The essential role of inorganic substrate in the migration and osteoblastic differentiation of mesenchymal stem cells." in: Journal of the mechanical behavior of biomedical materials, Vol. 59, pp. 353-65, (2016) (PubMed).

Jiang, Sun, Li, Hu, Zhou, Jiang, Xu: "Calycosin-7-O-?-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells." in: BMC complementary and alternative medicine, Vol. 15, pp. 315, (2015) (PubMed).

Li, Sun, Yang, Hu, Jiang: "Tanshinone II a protects against lipopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway." in: Chinese journal of integrative medicine, Vol. 20, Issue 3, pp. 216-23, (2014) (PubMed).

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin (VCL Products)

Synonyms: CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody

Background:

Synonyms: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, VCL

Background: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2