WWP1 antibody (AA 21-120)

Catalog No. ABIN720910

Product Details anti-WWP1 Antibody

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Binding Specificity: AA 21-120

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human WWP1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for WWP1

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Alternative Name: WWP1 (WWP1 Products)

Synonyms: AIP5 antibody, Tiul1 antibody, hSDRP1 antibody, 8030445B08Rik antibody, SDRP1 antibody, WWP1 antibody, aip5 antibody, tiul1 antibody, hsdrp1 antibody, WW domain containing E3 ubiquitin protein ligase 1 antibody, WWP1 antibody, Wwp1 antibody, wwp1 antibody

Background:

Synonyms: AIP 5, AIP5, Atropin-1-interacting protein 5, hSDRP1, nedd4 like, NEDD4-like E3 ubiquitin-protein ligase WWP1, Tiul1, WW domain containing E3 ubiquitin protein ligase 1, WW domain-containing protein 1, WWP 1, WWP1_HUMAN.

Background: WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.

Gene ID: 11059