His Tag antibody

Details for Product No. ABIN722438, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
620
18
14
3
1
Host
Mouse
412
181
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
59
45
36
15
13
11
10
9
9
8
8
8
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
545
386
164
106
77
76
68
26
12
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen KLH conjugated HHHHHH peptide
Sequence HHHHHH
Clone 2D5
Isotype IgG
Characteristics Antibody is specific for: HHHHHH tag
Purification Purified by Protein G.
Target Type Tag
Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Synonyms: HisX6, 6His, His Tag, 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Histidine.

Research Area Tags/Labels
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN722438) Lane 1-3: His Tagged protein lysates probed with His tag (2D5) Monoclonal Antibody a...
Product cited in: Li, Hu: "C5a of Cynoglossus semilaevis has anaphylatoxin-like properties and promotes antibacterial and antiviral defense." in: Developmental and comparative immunology, Vol. 60, pp. 139-48, 2016 (PubMed).

Gao, Han, Deng, Hu, Zhen, Li, Qin, Yan, Wu, Liu, Zhao, Pang: "The role of a novel C-type lectin-like protein from planarian in innate immunity and regeneration." in: Developmental and comparative immunology, Vol. 67, pp. 413-426, 2016 (PubMed).

Li, Li, Sun: "CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity." in: Scientific reports, Vol. 6, pp. 39287, 2016 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC)

Zhou, Sun: "CsCTL1, a teleost C-type lectin that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved EPN motif." in: Developmental and comparative immunology, Vol. 50, Issue 2, pp. 69-77, 2015 (PubMed).

Wang, Ren, Pan, Liu, Zhang: "Enhanced tolerance and remediation to mixed contaminates of PCBs and 2,4-DCP by transgenic alfalfa plants expressing the 2,3-dihydroxybiphenyl-1,2-dioxygenase." in: Journal of hazardous materials, Vol. 286, pp. 269-75, 2015 (PubMed).

Zhang, Zhang, Sun: "P247 and p523: two in vivo-expressed megalocytivirus proteins that induce protective immunity and are essential to viral infection." in: PLoS ONE, Vol. 10, Issue 3, pp. e0121282, 2015 (PubMed).

Zhang, Sun et al.: "Tongue sole (Cynoglossus semilaevis) prothymosin alpha: Cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and ..." in: Developmental and comparative immunology, Vol. 53, Issue 1, pp. 96-104, 2015 (PubMed).

Yin, Zhong, Wang, Zhao, Zhao, Kuang: "Tong Xie Yao Fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P." in: World journal of gastroenterology : WJG, Vol. 21, Issue 15, pp. 4536-46, 2015 (PubMed).

Sun, Sun: "A short-type peptidoglycan recognition protein from tongue sole (Cynoglossus semilaevis) promotes phagocytosis and defense against bacterial infection." in: Fish & shellfish immunology, Vol. 47, Issue 1, pp. 313-20, 2015 (PubMed).

Sun, Liu, Li, Sun: "Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria." in: Developmental and comparative immunology, 2015 (PubMed).

Li, Zhang: "CsTNF1, a teleost tumor necrosis factor that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved receptor binding site." in: Developmental and comparative immunology, Vol. 55, pp. 65-75, 2015 (PubMed).

Zhang, Zhang, Xiao, Sun: "Rock bream (Oplegnathus fasciatus) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection." in: Developmental and comparative immunology, Vol. 45, Issue 1, pp. 35-42, 2014 (PubMed).

Qin, Li, He, Yang, Xie, Hu, Chen, Wang, Pu, Liao: "Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor." in: Analytica chimica acta, Vol. 829, pp. 60-7, 2014 (PubMed).

Yu, Sun, Li, Sun: "Characterization of a c-type lysozyme of Scophthalmus maximus: expression, activity, and antibacterial effect." in: Fish & shellfish immunology, Vol. 34, Issue 1, pp. 46-54, 2012 (PubMed).

Sun, Zheng, Hu, Sun, Sun: "Edwardsiella tarda Eta1, an in vivo-induced antigen that is involved in host infection." in: Infection and immunity, Vol. 80, Issue 8, pp. 2948-55, 2012 (PubMed).

Li, Zhang, Wang, Sun: "A peptidoglycan recognition protein from Sciaenops ocellatus is a zinc amidase and a bactericide with a substrate range limited to Gram-positive bacteria." in: Fish & shellfish immunology, Vol. 32, Issue 2, pp. 322-30, 2011 (PubMed).

Did you look for something else?