Nestin antibody (AA 681-750)

Catalog No. ABIN723530

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 681-750

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Nestin

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Nestin antibody (ABIN723530)

Wang, Zhang, Chen, Xue, Guo, Liu, Zhao: "Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells." in: Scientific reports, Vol. 6, pp. 25946, (2018) (PubMed).

Kawashima, Shinkai, Suzuki: "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping." in: Dental materials journal, Vol. 35, Issue 3, pp. 479-89, (2016) (PubMed).

Liu, Xue, Li, Zhang, Fu, Feng, Chen, Xu: "Amelioration of stroke-induced neurological deficiency by lyophilized powder of catapol and puerarin." in: International journal of biological sciences, Vol. 10, Issue 4, pp. 448-56, (2014) (PubMed).

Shan, Zhou, Yang, Yan, Zhang, Fu, Jiang: "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/?-catenin signaling." in: Cell biology international, (2014) (PubMed).

Yamamizu, Fujihara, Tachibana, Katayama, Takahashi, Hara, Imai, Shinkai, Yamashita: "Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a." in: Cell stem cell, Vol. 10, Issue 6, pp. 759-70, (2012) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background:

Synonyms: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes, NEST_RAT, Nestin.

Background: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Gene ID: 25491

UniProt: P21263