GSK-3 Beta (NT) antibody

Antigen: GSK-3 Beta (NT)

Clonality: Polyclonal

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine), Pig (Porcine), Cow (Bovine), Horse (Equine), Sheep (Ovine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Catalog no.: ABIN723740

Additional Information

Gene ID: 2932

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human GSK-3 Beta
Please inquire for sequence information.

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Horse (Equine), Pig (Porcine), Sheep (Ovine), Dog (Canine), Chicken

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: glycogen synthase kinase 3 beta, GSK 3 beta, GSK 3B, GSK3B, GSK3B protein, GSK3beta isoform, GSK3 beta, Glycogen synthase kinase-3 beta, GSK-3 beta, GSK3B_HUMAN.

Molecular Weight: 47kDa

Comments:

Glycogen synthase kinase-3 beta (GSK3Beta) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms alpha (GSK3A MIM 606784) and beta show a high degree of amino acid homology (Stambolic and Woodgett 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism neuronal cell development and body pattern formation. Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain thereby reducing its affinity for DNA. Expressed in testis thymus prostate and ovary and weakly expressed in lung brain and kidney. Belongs to the Ser/Thr protein kinase family.

Application Details

Application Notes: WB(1:100-500),ELISA(1:500-1000),IP(1:20-100),IHC-P(1:100-500),IHC-F(1:100-500),IF(1:100-500),Not yet tested in other applications. Optimal working dilutions must be determined by the end user.

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA

Preservative: 0.1% (w/v) Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only