BNP antibody (Biotin)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), RBITC (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), Biotin (1)
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Application
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 Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. |
ABIN724102 |
| Quantity |
100 µg (1mg/1ml) (Variants) |
| Price |
290.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Immunogen
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Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human BNP C-terminus
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Format
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Lyophilized
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Reconstitution
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Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
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Isotype
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IgG
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Description
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Synonym: BNP 32, Brain natriuretic peptide 32, Gamma brain natriuretic peptide, Natriuretic peptide brain type, Natriuretic peptide precursor B, Natriuretic peptides B, NPPB, NPPB protein.
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Molecular Weight
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14kDa
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Comments
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Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones which includes atrial natriuretic peptide (ANP) BNP C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity_x000D_
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Application Notes
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WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)
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Concentration
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1mg/1ml
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Purification
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Antibodies are purified by protein A and peptide affinity chromatography.
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Buffer
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0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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Storage
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Ship at room temperature. Store at -20°C
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Research Area
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Cardiovascular, Hypertrophy, Hormones
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Restrictions
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For Research Use only
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Alternatives for antigen "BNP", type "Antibodies"
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Hosts
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Rabbit (44), Mouse (15), Goat (2)
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Reactivities
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Human (39), Mouse (Murine) (24), Rat (Rattus) (22), Pig (Porcine) (4), Cow (Bovine) (3), Dog (Canine) (3), Rabbit (2), Horse (Equine) (1)
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Applications
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Flow Cytometry (FACS) (41), Immunofluorescence (IF) (41), ELISA (13), Immunohistochemistry (IHC) (8), Western Blotting (WB) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (3), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), RBITC (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), Biotin (1)
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