Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

AKT 1/2/3 antibody (AA 401-479)

Reactivity: Human, Mouse, Rat, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN725195
  • Target See all AKT 1/2/3 products
    AKT 1/2/3
    Binding Specificity
    • 25
    • 23
    • 23
    • 23
    • 20
    • 20
    • 18
    • 18
    • 16
    • 16
    • 15
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 401-479
    Reactivity
    • 164
    • 152
    • 138
    • 45
    • 31
    • 28
    • 24
    • 15
    • 9
    • 6
    • 4
    • 4
    Human, Mouse, Rat, Cow, Pig
    Host
    • 162
    • 2
    Rabbit
    Clonality
    • 151
    • 13
    Polyclonal
    Conjugate
    • 41
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    This AKT 1/2/3 antibody is un-conjugated
    Application
    • 147
    • 92
    • 91
    • 90
    • 35
    • 31
    • 29
    • 20
    • 9
    • 7
    • 6
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Cow, Human, Mouse, Pig, Rat
    Predicted Reactivity
    Dog,Cow,Sheep,Chicken
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human AKT1
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029769 (Flow Cytometry)
    'Independent Validation' Badge
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029769
    Date
    07/22/2014
    Antigen
    Lot Number
    131113
    Method validated
    Flow Cytometry
    Positive Control
    MDA-MB-468 cells - lower expression
    Negative Control
    Notes
    A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
    • Catalog number: ABIN725195
    • Lot number: 131113
    • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
    Secondary Antibody
    • Antibody: Goat anti-rabbit IgG-FITC
    • Lot number: 115629
    • Dilution: 1:200 in 1X PBS containing 0.5% BSA
    Full Protocol
    • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
    • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
    • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
    • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
    • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
    • Cells were incubated for 30 min on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
    • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
    • Labeled cells were washed twice in PBS containing 0.5% BSA.
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
    Experimental Notes
    Nothing to note.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Liu, Liu, Liu, Ma, Zhao: "Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala." in: Molecular pain, Vol. 14, pp. 1744806918805902, (2019) (PubMed).

    Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

    Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, (2016) (PubMed).

    Zhang, Huang, Guo, Gou, Hinsdale, Lloyd, Liu: "MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5404-14, (2015) (PubMed).

    Liang, Zeng, Wang, Shen, Li, Ma, Ci, Yu, Jia, Sun, Liu, Liu, Li, Yu, Chen, Jia: "Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells." in: Oncotarget, Vol. 5, Issue 14, pp. 5798-807, (2015) (PubMed).

    Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, (2014) (PubMed).

    Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, (2013) (PubMed).

    Tang, Liu, Dong, Li, Li, Lin, Zheng, Hou, Li: "The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 820695, (2013) (PubMed).

  • Target
    AKT 1/2/3
    Alternative Name
    AKT1/2/3 (AKT 1/2/3 Products)
    Background

    Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG

    Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

    Gene ID
    207
    UniProt
    P31749
You are here:
Support