RAGE antibody (AA 151-250) (FITC)

Catalog No. ABIN725907

Product Details anti-RAGE Antibody (FITC)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Binding Specificity: AA 151-250

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAGE antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat AGER

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-RAGE antibody (FITC) (ABIN725907)

Zhu, Yao, Zhang, Dong, Yu, Sheng: "Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function." in: Acta pharmacologica Sinica, Vol. 35, Issue 9, pp. 1167-76, (2014) (PubMed).

Braach, Buschmann, Pflaum, Hudalla, Koch, Ryschich, Poeschl, Frommhold: "Anti-inflammatory functions of protein C require RAGE and ICAM-1 in a stimulus-dependent manner." in: Mediators of inflammation, Vol. 2014, pp. 743678, (2014) (PubMed).

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE (AGER Products)

Synonyms: RAGE antibody, AGER antibody, advanced glycosylation end-product specific receptor antibody, advanced glycosylation end product-specific receptor antibody, MAPK/MAK/MRK overlapping kinase antibody, AGER antibody, Ager antibody, LOC719012 antibody

Background:

Synonyms: RAGE, Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, Ager

Background: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S1A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides (By similarity). Interaction with S1B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

Gene ID: 81722

UniProt: Q63495

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins