14-3-3 (Alpha/Beta/Gamma/Delta/Epsilon) antibody (Biotin)

Antigen: 14-3-3 (Alpha/Beta/Gamma/Delta/Epsilon)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine), Sheep (Ovine), Fruit Fly (Drosophila melanogaster)

Conjugate: Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN726712

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human 14-3-3 C-terminus

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: 14 3 3 protein beta, 14 3 3 protein beta/alpha, 14 3 3 protein zeta, KCIP 1, Protein 1054, Protein kinase C inhibitor protein 1, 14 3 3 protein delta, 14 3 3 protein gamma, YWHAB, YWHAZ, 14 3 3 protein Epsilon.

Molecular Weight: 31kDa

Comments:

14-3-3 are activates tyrosine and tryptophan hydroxylases in the presence of Ca (2+)/calmodulin-dependent protein kinase II

and strongly activates protein kinase C. Is probably a multifunctional regulator of the cell signaling processes mediated by both kinases. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin. 14-3-3 proteins are localized in neurons

and are axonally transported to the nerve terminals. They may be also present

at lower levels

in various other eukaryotic tissues. It belongs to the 14-3-3 family.. This antibody is reactive with 14-3-3 Alpha/Beta/Gamma/Delta/Epsilon.

Application Details

Application Notes: WB (1:100-500), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only