PAR1 antibody (AA 251-350)

Catalog No. ABIN728743

Product Details anti-PAR1 Antibody

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Binding Specificity: AA 251-350

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PAR1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PAR1 antibody (ABIN728743)

Posthuma, Posma, Schep, Bender, van Oerle, van der Wal, Ten Cate, Spronk: "Protease-activated receptors are potential regulators in the development of arterial endofibrosis in high-performance athletes." in: Journal of vascular surgery, Vol. 69, Issue 4, pp. 1243-1250, (2019) (PubMed).

Ben Shimon, Zeimer, Shavit Stein, Artan-Furman, Harnof, Chapman, Eisenkraft, Pick, Maggio: "Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus." in: PLoS ONE, Vol. 12, Issue 11, pp. e0188524, (2017) (PubMed).

Fan, Chen, Ni, Wang, Sun, Chen, Zheng: "Expression of MMP-1/PAR-1 and patterns of invasion in oral squamous cell carcinoma as potential prognostic markers." in: OncoTargets and therapy, Vol. 8, pp. 1619-26, (2015) (PubMed).

Acton, Miles: "Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134488, (2015) (PubMed).

Liu, Xie, Luo, Chen, Zhou, Xia, Chen, Zhou, Cao, Cao, Zhou: "Identification of FLOT2 as a novel target for microRNA-34a in melanoma." in: Journal of cancer research and clinical oncology, (2014) (PubMed).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: Thrombin receptor (F2R Products)

Synonyms: CF2R antibody, HTR antibody, PAR-1 antibody, PAR1 antibody, TR antibody, Par1 antibody, TRGPC antibody, AI482343 antibody, Cf2r antibody, ThrR antibody, Par-1 antibody, f2r-a antibody, par1 antibody, coagulation factor II thrombin receptor antibody, coagulation factor II (thrombin) receptor antibody, coagulation factor 2 (thrombin) receptor L homeolog antibody, F2R antibody, F2r antibody, f2r.L antibody

Background:

Synonyms: TR, HTR, CF2R, PAR1, PAR-1, Proteinase-activated receptor 1, Coagulation factor II receptor, Thrombin receptor, F2R

Background: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Gene ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus