F-Actin antibody (Cy5)
| Antigen |
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
 Alternatives Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Sheep (Ovine), Pig (Porcine)
|
|
Conjugate
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Application
|
|
| Catalog no. |
ABIN731502 |
| Quantity |
100 µg (1mg/1ml) |
| Price |
330.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 10 to 15 Business Days |
|
Immunogen
|
Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human F-Actin
|
|
Format
|
Lyophilized
|
|
Reconstitution
|
Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
|
|
Isotype
|
IgG
|
|
Description
|
Synonym: f actin, Filamentous actin.
|
|
Specificity
|
Excitation / Emission Wavelengths: 650nm/670nm
|
|
Molecular Weight
|
42kDa
|
|
Comments
|
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells_x000D_ .G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling in which filament length remains constant and actin monomers add at one end and dissociated at the other.
|
|
Application Notes
|
Flow-Cyt (1:50-200), IF (1:50-200)
|
|
Concentration
|
1mg/1ml
|
|
Purification
|
Antibodies are purified by protein A and peptide affinity chromatography.
|
|
Buffer
|
0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
|
|
Storage
|
Ship at room temperature. Store at -20°C
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "F-Actin", type "Antibodies"
|
Hosts
|
Rabbit (19), Mouse (2)
|
|
Reactivities
|
Human (21), Mouse (Murine) (19), Rat (Rattus) (19), Cow (Bovine) (15), Pig (Porcine) (14), Sheep (Ovine) (14), Chicken (1), Dog (Canine) (1)
|
|
Applications
|
Flow Cytometry (FACS) (19), Immunofluorescence (IF) (17), ELISA (4), Western Blotting (WB) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
