Elastin (ELN) (AA 750-786) antibody

Details for Product No. ABIN734003, Supplier: Log in to see
Antigen
  • ELN
  • SVAS
  • WBS
  • WS
  • RATTREL11
  • TREL11
  • Trela
  • Trela26
  • AI385707
  • AI480567
  • E030024M20Rik
  • elastin
  • elastin (supravalvular aortic stenosis, Williams-Beuren syndrome)
  • elastin-like
  • ELN
  • Tcur_2481
  • Kfla_2289
  • Eln
  • LOC100620140
Alternatives
anti-Rat (Rattus) Elastin antibody for ELISA
Epitope
AA 750-786
8
6
5
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
66
22
18
15
13
13
5
4
3
3
3
3
1
Host
Rabbit
57
29
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
56
42
29
26
14
11
10
9
8
3
2
2
1
Options
Supplier
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Available images

'Independent Validation' Badge
Lot Number 120306
Method validated Immunohistochemistry
Positive Control Mouse heart tissue
Negative Control Mouse adipose tissue
Notes Signal was detected in positive control tissue and not detected in negative control tissue.
Primary Antibody
  • Antibody: Elastin (ELN) (C-Term) antibody
  • Catalog number: ABIN734003
  • Lot number: 120306
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: Wild type mouse heart tissue (specimen known to contain the target protein).
  • Negative control: Wild type adipose tissue (specimen known to not contain the target protein).
  • Primary antibody isotype control: Wild type mouse heart treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse heart treated with secondary antibody only (no
  • primary antibody).
Protocol
  • Immunohistochemistry was performed manually.
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
  • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
  • Sections were washed x 3 in Tris buffered saline (TBS).
  • Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
  • Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
  • Sections were washed x 3 in Tris buffered saline.
  • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
  • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin for 1 min.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
Experimental Notes None
Validation Images
Immunohistochemistry validation image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue...
Immunogen KLH conjugated synthetic peptide derived from human Elastin
Isotype IgG
Purification Purified by Protein A.
Alternative Name Elastin (ELN Antibody Abstract)
Background

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Subcellular location: Extracellular, Secreted

Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN

Gene ID 2006
UniProt P15502
Research Area Cardiovascular, Extracellular Matrix
Pathways Regulation of Actin Filament Polymerization
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclona...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclona...
SDS-PAGE (SDS) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclo...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTI...
Immunohistochemistry (IHC) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Images provided the Independent Validation Program (badge number 28751): Formalin-fix...
Flow Cytometry (FACS) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody
Western Blotting (WB) image for anti-Elastin (ELN) (AA 750-786) antibody (ABIN734003) Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated at 1:300 ov...
Product cited in: Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, 2014 (PubMed).

Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." in: Hypertension, Vol. 62, Issue 5, pp. 942-50, 2013 (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, 2012 (PubMed).

Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." in: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, 1970 (PubMed).