Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody (Gold)

Antigen: Lymphoid Enhancer-Binding Factor 1 (LEF1)

Synonyms: FLJ46390, TCF1ALPHA, DKFZp586H0919, LEF-1, LEF1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine), Rabbit, Chicken

Conjugate: Gold

Application: Immunoelectron Microscopy (IEM)

Catalog no.: ABIN735256

Additional Information

Alternative name: LEF-1

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human LEF-1

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: lymphoid enhancing factor-1, Transcription factor T cell specific 1 alpha, DKFZp586H0919, LEF 1, LEF1, Lymphoid enhancer binding factor 1, T cell specific transcription factor 1 alpha, TCF 1 alpha, TCF1 alpha, TCF1alpha.

Molecular Weight: 44kDa

Comments:

Lymphoid enhancer factor

LEF1

is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF1

TCF1

TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling

a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer

melanoma and breast cancer. . LEF1 is expressed during development in many different differentiating tissues

and in a few tissues after birth. LEF1 expression is required for proper development of breast

teeth

hair

whiskers and the trigeminal nerve. It is redundant with TCF1 (for T cell Factor 1) for correct development of T lymphocytes in the thymus. LEF1 is a 399 amino acid protein. Alternative splice forms of LEF1 and other LEF/TCF family members have been identified

but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on immunoblots as small immunoreactive forms.

Application Details

Application Notes: Electron Microscopy (1:100-500)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only