ENO2/NSE antibody (AA 201-300)

Catalog No. ABIN736581

Product Details anti-ENO2/NSE Antibody

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Binding Specificity: AA 201-300

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NSE

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ENO2/NSE antibody (ABIN736581)

Yan, Bai, Lu, Gao, Bi, Lv: "Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage." in: Iranian journal of basic medical sciences, Vol. 20, Issue 9, pp. 1029-1036, (2017) (PubMed).

Huang, Li, Huang, Xie, Xu, Chen, Gao: "A novel reverse fluorescent immunoassay approach for sensing human chorionic gonadotropin based on silver-gold nano-alloy and magnetic nanoparticles." in: Analytical and bioanalytical chemistry, Vol. 408, Issue 2, pp. 619-27, (2016) (PubMed).

Zhang, Li, Lu, Liu et al.: "Cerebral potential biomarkers discovery and metabolic pathways analysis of ?-synucleinopathies and the dual effects of Acanthopanax senticosus Harms on central nervous system through metabolomics ..." in: Journal of ethnopharmacology, Vol. 163, pp. 264-72, (2015) (PubMed).

Wang, Xu, Qin, Wang, Ma, Luo, Zhang: "Myocardin-related transcription factor-A is a key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells." in: Gene, Vol. 523, Issue 2, pp. 178-86, (2013) (PubMed).

Li, Tian: "Guanine-decorated graphene nanostructures for sensitive monitoring of neuron-specific enolase based on an enzyme-free electrocatalytic reaction." in: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Vol. 29, Issue 12, pp. 1195-201, (2013) (PubMed).

Yu, Cheng, Li, Luo, Yan, Zhang, Yin, Ding, Ju: "Electrochemical immunosensor for competitive detection of neuron specific enolase using functional carbon nanotubes and gold nanoprobe." in: Talanta, Vol. 93, pp. 433-8, (2012) (PubMed).

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: NSE (ENO2 Products)

Background:

Synonyms: NSE, HEL-S-279, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, ENO2

Background: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Gene ID: 2026

UniProt: P09104