IRF7 antibody (pSer471, pSer472)

Catalog No. ABIN742703

Product Details anti-IRF7 Antibody

Target: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Binding Specificity: pSer471, pSer472

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IRF7 antibody (ABIN742703)

Le Bel, Gosselin: "Leukotriene B4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139856, (2015) (PubMed).

Han, Choi, Uyangaa, Kim, Kim, Kim, Kim, Eo: "Distinct dictation of Japanese encephalitis virus-induced neuroinflammation and lethality via triggering TLR3 and TLR4 signal pathways." in: PLoS pathogens, Vol. 10, Issue 9, pp. e1004319, (2014) (PubMed).

Menasria, Boivin, Lebel, Piret, Gosselin, Boivin: "Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection." in: Journal of virology, Vol. 87, Issue 13, pp. 7301-8, (2013) (PubMed).

Target Details for IRF7

Target: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Alternative Name: IRF7 + (IRF7 Products)

Synonyms: wu:fl24c03 antibody, zgc:66043 antibody, zgc:77947 antibody, IRF7 antibody, irf7 antibody, IRF-7H antibody, IRF7A antibody, IRF7B antibody, IRF7C antibody, IRF7H antibody, interferon regulatory factor 7 antibody, irf7 antibody, IRF7 antibody, Irf7 antibody

Background:

Synonyms: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7, IRF7

Background: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.

Gene ID: 3665

UniProt: Q92985

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagy