PAK2 antibody (pSer20)

Catalog No. ABIN744518

Product Details anti-PAK2 Antibody

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Binding Specificity: pSer20

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PAK2 around the phosphorylation site of Ser20

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PAK2

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Alternative Name: PAK2 (PAK2 Products)

Synonyms: PAK65 antibody, PAKgamma antibody, gamma-PAK antibody, 5330420P17Rik antibody, A130002K10Rik antibody, AI836325 antibody, D16Ertd269e antibody, PAK-2 antibody, mKIAA4182 antibody, pak2 antibody, cb422 antibody, kinase antibody, zgc:91798 antibody, wu:fb71h01 antibody, si:dz198m22.1 antibody, pak65 antibody, pakgamma antibody, PAKI antibody, Pak3 antibody, CH73-159L23.1 antibody, wu:fc52b03 antibody, zgc:113857 antibody, p21 (RAC1) activated kinase 2 antibody, p21 protein (Cdc42/Rac)-activated kinase 2 antibody, p21 protein (Cdc42/Rac)-activated kinase 2a antibody, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog antibody, p21 protein (Cdc42/Rac)-activated kinase 2b antibody, PAK2 antibody, Pak2 antibody, pak2a antibody, pak2.S antibody, pak2b antibody

Background:

Synonyms: PAK2 phospho S20, PAK2 phospho Ser20, p-PAK2 S20, p-PAK2 Ser20, CB422, EC 2.7.11.1, Gamma PAK, hPAK65, Kinase, p21 CDKN1A-activated kinase 2a, p21 activated kinase 2, p21 protein Cdc42/Rac-activated kinase 2, p21 protein Cdc42 Rac activated kinase 2, p21-activated kinase, 65-KD antibody p21-activated protein kinase I, p21CDKN1A activated kinase 2, p58, p65PAK, PAK 2, PAK65, PAKgamma, S6 H4 kinase, Serine threonine protein kinase PAK 2.

Background: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Gene ID: 5062

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha