RPS6KA2 antibody (pThr353, pThr356)

Catalog No. ABIN745238

Product Details anti-RPS6KA2 Antibody

Target: RPS6KA2 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 2 (RPS6KA2))

Binding Specificity: pThr353, pThr356

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RSK3 around the phosphorylation site of Thr353/356

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPS6KA2

Target: RPS6KA2 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 2 (RPS6KA2))

Alternative Name: RSK3 + (RPS6KA2 Products)

Synonyms: HU-2 antibody, MAPKAPK1C antibody, RSK antibody, RSK3 antibody, S6K-alpha antibody, S6K-alpha2 antibody, p90-RSK3 antibody, pp90RSK3 antibody, 90kDa antibody, D17Wsu134e antibody, Rps6ka-rs1 antibody, Rsk3 antibody, p90rsk antibody, pp90rsk antibody, ribosomal protein S6 kinase A2 antibody, ribosomal protein S6 kinase, polypeptide 2 antibody, Rps6ka2 antibody, RPS6KA2 antibody

Background:

Synonyms: 90 kDa ribosomal protein S6 kinase 2, HU 2, KS6A2_HUMAN, MAP kinase activated protein kinase 1c, MAP kinase-activated protein kinase 1c, MAPK-activated protein kinase 1c, MAPKAP kinase 1c, MAPKAPK-1c, MAPKAPK1C, p90-RSK 2, p90RSK 2, p90RSK2, pp90RSK 3, pp90RSK3, Ribosomal protein S6 kinase alpha-2, Ribosomal S6 kinase 3, RPS6KA2, RSK 3, RSK-3, S6K alpha 2, S6K alpha, S6K-alpha-2.

Background: RSK3(Ribosomal S6 kinase 3)is a member of a family of 90- kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases that are activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2. Rsk3 is a distinct isoform of p90Rsk that translocates to the cell nucleus, phosphorylates potential nuclear targets and may have a unique upstream activator. Several sites, such as Ser380, Thr359, Ser363 and Thr573, are important for its activation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.

Gene ID: 6196

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Activation of Innate immune Response, Toll-Like Receptors Cascades