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KAP1 antibody (pSer824)

TRIM28 Reactivity: Human WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN746108
  • Target See all KAP1 (TRIM28) Antibodies
    KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
    Binding Specificity
    • 28
    • 15
    • 11
    • 10
    • 8
    • 6
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer824
    Reactivity
    • 113
    • 53
    • 49
    • 10
    • 9
    • 8
    • 7
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 114
    • 15
    • 4
    Rabbit
    Clonality
    • 107
    • 26
    Polyclonal
    Conjugate
    • 62
    • 10
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This KAP1 antibody is un-conjugated
    Application
    • 85
    • 42
    • 40
    • 26
    • 25
    • 22
    • 22
    • 20
    • 19
    • 15
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This phosphorylation site is homologous across the listed species.
    Cross-Reactivity
    Human
    Predicted Reactivity
    Mouse,Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human TIF1 beta around the phosphorylation site of Ser824
    Isotype
    IgG
    Top Product
    Discover our top product TRIM28 Primary Antibody
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Ding, Zhang, Wang, Wang, Fan, He, Zhang, Gao, Li, Chen: "γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes." in: Scientific reports, Vol. 6, pp. 38295, (2016) (PubMed).

  • Target
    KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
    Alternative Name
    T1 beta (TRIM28 Products)
    Synonyms
    KAP1 antibody, RNF96 antibody, TF1B antibody, TIF1B antibody, TRIM28 antibody, kap1 antibody, rnf96 antibody, tf1b antibody, tif1b antibody, AA408787 antibody, KAP-1 antibody, KRIP-1 antibody, Tif1b antibody, Tif1beta antibody, Kap1 antibody, Krip1 antibody, TIF1BETA antibody, tripartite motif containing 28 antibody, tripartite motif containing 28 L homeolog antibody, tripartite motif-containing 28 antibody, TRIM28 antibody, trim28.L antibody, Trim28 antibody
    Background

    Synonyms: KAP1, TF1B, RNF96, TIF1B, PPP1R157, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, TRIM28

    Background: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation, the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors.

    Gene ID
    10155
    UniProt
    Q13263
    Pathways
    Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
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