Angiotensin III (AGT3) antibody

Antigen: Angiotensin III (AGT3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Horse (Equine)

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN750508

Additional Information

Alternative name: Angiotensin III

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human Angiotensin III

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG  (Matching secondary antibodies)

Description: Synonym: Ang III, Angiotensin 3, Angiotensin3, Angiotensin 3, Angiotensinogen, ANHU, SERPINA8.

Comments:

Angiotensin III is an eptapeptide drived from cleavage of full length angiotensin (AGT). AGT is a polypeptide vasopressor hormone formed by the catalytic action of renin

which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II

which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex

and also functions as a neurotransmitter. Angiotensin III (heptapeptide) is a degradation product of angiotensin II

and has less vasopressor activity than the parent compound. However

Angiotensin III does stimulate aldosterone release.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Cancer, Endocrine system

Restrictions: For Research Use only