GLUT5 antibody (HRP)

Antigen: GLUT5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN752167

Additional Information

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human GLUT5

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: GLUT-5, GLUT 5, Glucose Transporter 5 Facilitated glucose transporter member 5, Fructose transporter, Glucose transporter type 5 small intestine, SLC2A5, Solute carrier family 2, small intestine.

Molecular Weight: 55kDa

Comments:

Ten types of glucose/fructose transport carrier proteins designated as Glut 1-10 facilitate glucose/fructose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. Majority of the differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue-specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers.
_x000D_ Glut 5 seems to function primarily as a fructose transporter

it is expressed in small intestine

and at much lower levels in kidney

skeletal muscle

and adipose

Application Details

Application Notes: WB (1:200-1000), ELISA (1:500-1000)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Restrictions: For Research Use only