BRCA1 antibody (pSer1189) (Cy5.5)

Catalog No. ABIN756642

Product Details anti-BRCA1 Antibody (Cy5.5)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Binding Specificity: pSer1189

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BRCA1 around the phosphorylation site of Ser1189

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 (BRCA1 Products)

Synonyms: BRCAI antibody, BRCC1 antibody, BROVCA1 antibody, IRIS antibody, PNCA4 antibody, PPP1R53 antibody, PSCP antibody, RNF53 antibody, iris antibody, pscp antibody, brcai antibody, brcc1 antibody, rnf53 antibody, brca1 antibody, BRCA1 antibody, LOC100224649 antibody, BRCA1, DNA repair associated antibody, breast cancer 1 antibody, breast cancer 1, early onset antibody, breast cancer 1 L homeolog antibody, BRCA1 antibody, brca1 antibody, LOC755086 antibody, Brca1 antibody, brca1.L antibody

Background:

Synonyms: BRCA1 phospho Ser1189, BRCA1 phospho S1189, p-BRCA1 phospho S1189, BRCA 1, BRCA1, BRCA1/BRCA2 containing complex subunit 1, BRCA1/BRCA2-containing complex, subunit 1, BRCA1_HUMAN, BRCAI, BRAC 1, BRCA 1, BRCC 1, BRCC1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast cancer type 1 susceptibility protein, Breast and ovarian cancer susceptibility protein 1, Breast Ovarian Cancer Susceptibility, IRIS, Papillary Serous Carcinoma Of The Peritoneum, PSCP, RING finger protein 53, BROVCA1, IRIS, PNCA4, PPP1R53, Protein phosphatase 1 regulatory subunit 53, RNF53, BAP1.

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009].

Gene ID: 672

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus