ADAM Metallopeptidase Domain 15 (ADAM15) antibody (Cy7)

Antigen: ADAM Metallopeptidase Domain 15 (ADAM15)

Synonyms: MDC15, metargidin, tMDCVI, ADAM15, mdc15

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Cy7

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN758777

Additional Information

Alternative name: ADAM15

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human ADAM15

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: A disintegrin and metalloproteinase domain 15, A disintegrin and metalloproteinase domain 15, ADAM metallopeptidase domain 15, MDC15, Metalloprotease RGD disintegrin protein, Metalloproteinase like disintegrin like and cysteine rich protein 15, Metargidin.

Specificity: Excitation / Emission Wavelengths: 743nm/767nm

Comments:

ADAM15

also known as Metargidin and MDC15

is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It was first described in mammary epithelial carcinoma cells as a membrane anchored Metalloprotease Disintegrin with RGD sequence (MetaRGiDin). Mouse ADAM15 does not contain the RGD sequence

instead the mouse ADAM15 has a TDD in its place. Like ADAM9 and ADAM12

there are SH3 ligand domains in the cytoplasmic portion of ADAM15

suggesting regulation routes for ADAM15 via Src and Src tyrosine kinase. ADAM15 contains the canonical HExxHxxxxxH zinc metalloproteinase motif

and has been shown to be proteolytically active. Full length ADAM15

814 amino acids

has a predicted mass of 85 kDa

but due to glycosylation and cysteine rich regions

the reduced protein migrates to 110 kDa (unprocessed) and 84 kDa (processed). ADAM15 is widely expressed in normal tissues and tumor cell lines

and is expressed on the cell surface

anchored by the transmembrane domain. It is also elevated in osteoarthritic and rheumatoid synovial tissues

suggesting an inflammatory role. ADAM15 may also act as a cell attachment molecule by binding the integrins avbeta3 and a5beta1 through the cysteine rich domain and/or RGD sequence.

Application Details

Application Notes: Flow-Cyt (1:50-200), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix

Restrictions: For Research Use only