ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 2 (Adamts2) antibody (Biotin)

Antigen: ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 2 (Adamts2)

Synonyms: NPI, PCINP, PCPNI, hPCPNI, ADAM-TS2, ADAMTS-3, METH2, ADAM-TS8, FLJ41712, ADMP-2, ADAMTS11, FLJ36738, ADAM-TS7, DKFZp434H204, ADAMTS7B, ADAMTS5, ADAMTS-11

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN758923

Additional Information

Alternative name: ADAMTS2

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human ADAMTS2

Format: Lyophilized

Reconstitution: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotype: IgG

Description: Synonym: A disintegrin and metalloproteinase with thrombospondin motifs 2, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2, ADAM metallopeptidase with thrombospondin type 1 motif 2, ADAM TS 2, ADAM TS2, ADAMTS2, ADAMTS 2, ADAMTS-2, ADAMTS 3, ADAMTS3, ADAMTS-3, EDS VIIB, EDS VIIC, hPCPNI, NPI, PC I NP, PCINP, PCPNI, pNPI, Procollagen I N proteinase, Procollagen I/II amino propeptide processing enzyme, Procollagen N endopeptidase.

Comments:

ADAMTS2 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS2 (A Disintegrin And Metalloproteinase with ThromboSpondin-2 motif)

also known as Procollagen I N-Proteinase (PNP)

was first described in calf skin as a proteinase that processes the amino end of Type-I collagen. PNP expression was found in skin

aorta

liver

tendon

bladder

retina

and skeletal muscle. Later

PNP was found to be a member of a larger family of ADAMs metalloproteinases containing thrombospondin (TS) repeats. Full length human ADAMTS2 contains 1211 amino acids (bovine

1205 amino acids) and has a predicted mass of 134.7 kDa

but glycosylation and the abundance of cysteine residues gives ADAMTS2 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS2 resolves at a lower molecular weight of 107 kDa

due to cleavage at the furin site. ADAMTS2 contains the canonical HexxHxxxxxH zinc metalloproteinase motif

and has been shown to be proteolytically active

cleaving procollagen. In addition to the metalloprotease domain

ADAMTS2 has a propeptide domain

a prohormone convertase (PC

furin) cleavage site

a cysteine-rich domain

and three thrombospondin 1 like domains

followed by a unique C-terminal domain. ADAMTS2 does not have a transmembrane domain

unlike many of the ADAMs proteases

and is a secreted protein

much of which binds to the ECM (extracellular matrix). ADAMTS2 knockout mice develop fragile skin (similar to dermatospaxis)

and male infertility. Mutations of the ADAMTS2 gene are responsible for human Ehlers-Danlos syndrome type VII C and bovine dermatosparaxis. ADAMTS2 is involved in collagen biosynthesis and may also play role in development and angiogenesis.

Application Details

Application Notes: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Concentration: 1mg/1ml

Purification: Antibodies are purified by protein A and peptide affinity chromatography.

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Storage: Ship at room temperature. Store at -20°C

Research Area: Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Angiogenesis, Matrix Metalloproteinases

Restrictions: For Research Use only