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WNT2 antibody (AA 221-320)

WNT2 Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN762896
  • Target See all WNT2 Antibodies
    WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
    Binding Specificity
    • 14
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 221-320
    Reactivity
    • 31
    • 21
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 30
    • 1
    Rabbit
    Clonality
    • 30
    • 1
    Polyclonal
    Conjugate
    • 17
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This WNT2 antibody is un-conjugated
    Application
    • 29
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity
    Human, Mouse
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human WNT2
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029629 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Histopathology and Tissue Shared Resource, Georgetown Lombardi Comprehensive Cancer Center
    No.
    #029629
    Date
    03/16/2014
    Antigen
    Lot Number
    131105
    Method validated
    Immunohistochemistry
    Positive Control
    Human glioma tissue
    Negative Control
    Human brain
    Notes
    Signal was detected in positive control tissue and not in negative control tissue.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
    • Catalog number: ABIN762896
    • Lot number: 131105
    Secondary Antibody
    • Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
    • Batch number: 10082183
    Full Protocol
    • Immunohistochemistry was performed by hand.
    • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
    • Sections were rinsed in de-ionized water for 5 min at RT.
    • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
    • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
    • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
    • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
    • Sections were rinsed in TBST for 5 min 2X at RT.
    • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
    • Sections were rinsed in TBST for 5 min 2X at RT.
    • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
    • Sections were washed x 1 in Distilled Water.
    • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were blued in Ammonium Hydroxide for 1 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
    Experimental Notes
    • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
    • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

  • Target
    WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
    Alternative Name
    WNT2 (WNT2 Products)
    Synonyms
    CG1916 antibody, D-wnt-2 antibody, DWnt-2 antibody, DWnt2 antibody, Dm DWnt2 antibody, Dm-2 antibody, Dmel\\CG1916 antibody, Dwnt-2 antibody, Wnt antibody, Wnt-2 antibody, dWnt2 antibody, wnt2 antibody, WNT2 antibody, irp antibody, Xwnt2 antibody, wnt-2 antibody, Xwnt-2 antibody, int1l1 antibody, 2610510E18Rik antibody, Int1l1 antibody, Irp antibody, Mirp antibody, Wnt2a antibody, INT1L1 antibody, IRP antibody, ZfWnt2 antibody, Wnt oncogene analog 2 antibody, Wnt family member 2 antibody, wingless-type MMTV integration site family member 2 antibody, wingless-type MMTV integration site family, member 2 antibody, Wnt2 antibody, wnt2 antibody, WNT2 antibody
    Background

    Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2

    Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

    Gene ID
    7472
    UniProt
    P09544
    Pathways
    WNT Signaling
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