MTHFD1L antibody (AA 535-538)

Catalog No. ABIN768655

Product Details anti-MTHFD1L Antibody

Target: MTHFD1L (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1-Like (MTHFD1L))

Binding Specificity: AA 535-538

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MTHFD1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MTHFD1L (aa535-538)

Sequence: DTNDRFLRKI TIGQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DTNDRFLRKITIGQ, from the internal region of the protein sequence according to NP_056255.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 110 kDa band observed in Human Brain (Cerebellum) (calculated MW of 106 kDa according to NP_056255.2). The same band is observed in lysates of cell line NIH3T3, Mouse Brain and Rat Brain. Recommended concentration: 0.1-0.3 μg/mL. Prima

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MTHFD1L

Target: MTHFD1L (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1-Like (MTHFD1L))

Alternative Name: MTHFD1L (MTHFD1L Products)

Background: MTHFD1L, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, dJ292B18.2, 10-formyl-THF synthetase, formyltetrahydrofolate synthetase domain containing 1, mitochondrial C1-tetrahydrofolate synthase

Molecular Weight: 106kDa according to NP_056255.2

Gene ID: 25902, 270685, 361472

NCBI Accession: NP_056255