PGK1 antibody (AA 102-151)

Catalog No. ABIN6747118

Product Details anti-PGK1 Antibody

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Binding Specificity: AA 102-151

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PGK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Horse, Guinea pig (100%) Pig, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa102-151 of human PGK1 (P00558, NP_000282). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Hamster, Elephant, Pig, Opossum, Zebra finch, Chicken, Platypus, Xenopus, Salmon, Stickleback, Zebrafish, Mosquito (92%), Medaka, Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1/Phosphoglycerate Kinase 1 (PGK1 Products)

Background: Name/Gene ID: PGK1
Subfamily: PGK
Family: Non-protein Kinase

Synonyms: PGK1, MIG10, Primer recognition protein 2, PRP 2, PGKA, Phosphoglycerate kinase 1

Gene ID: 5230

NCBI Accession: NP_000282

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process