alpha Defensin 1 antibody (AA 50-63)

Catalog No. ABIN769054

Product Details anti-alpha Defensin 1 Antibody

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Binding Specificity: AA 50-63

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Defensin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DEFA1. This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DESLAPKHPGSRKN from an internal region of human DEFA1 (NP_004075.1, NP_005208.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 10  kDa band observed in Human Spleen lysates (calculated MW of 10.2  kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 20  kDa. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Alternative Name: DEFA1 / HP1 (DEFA1 Products)

Synonyms: DEF1 antibody, DEFA2 antibody, HNP-1 antibody, HP-1 antibody, HP1 antibody, MRS antibody, Defcr antibody, Defcr1 antibody, DEFA1 antibody, Defa antibody, Def antibody, GB19392 antibody, defensin alpha 1 antibody, defensin, alpha 1 antibody, defensin alpha 5 antibody, alpha-defensin 1A antibody, defensin 1 antibody, neutrophil defensin 1 antibody, DEFA1 antibody, Defa1 antibody, Defa5 antibody, MNP1A antibody, Def1 antibody, LOC107973256 antibody

Background: Name/Gene ID: DEFA1

Synonyms: DEFA1, DEFA2, Defensin, alpha 2, HP-1, HP1, Myeloid-related sequence, MRS, DEF1, Defensin, alpha 1, HNP-1, Neutrophil defensin 1

Gene ID: 1667

NCBI Accession: NP_004075, NP_005208

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin