CD90 antibody (FITC)

Catalog No. ABIN780367

Product Details anti-CD90 Antibody (FITC)

Target: CD90 (THY1)

Reactivity: Human, Pig, Horse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons.

No Cross-Reactivity: Dog

Cross-Reactivity (Details): Human, Non-Human Primates, Porcine, Equine (Horse)

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HEL erythroleukemia cells

Clone: 5E10

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD90 antibody (FITC) (ABIN780367)

Emadedin, Labibzadeh, Fazeli, Mohseni, Hosseini, Moghadasali, Mardpour, Azimian, Goodarzi, Ghorbani Liastani, Mirazimi Bafghi, Baghaban Eslaminejad, Aghdami: "Percutaneous Autologous Bone Marrow-Derived Mesenchymal Stromal Cell Implantation Is Safe for Reconstruction of Human Lower Limb Long Bone Atrophic Nonunion." in: Cell journal, Vol. 19, Issue 1, pp. 159-165, (2017) (PubMed).

Labibzadeh, Emadedin, Fazeli, Mohseni, Hosseini, Moghadasali, Mardpour, Azimian, Ghorbani Liastani, Mirazimi Bafghi, Baghaban Eslaminejad, Aghdami: "Mesenchymal Stromal Cells Implantation in Combination with Platelet Lysate Product Is Safe for Reconstruction of Human Long Bone Nonunion." in: Cell journal, Vol. 18, Issue 3, pp. 302-309, (2016) (PubMed).

Barale, Dentelli, Togliatto, Trombetta, Olgasi, Scozzari, Toppino, Morino, Brizzi: "High glucose via NOX-dependent ROS generation and AKT activity promotes adipose-derived stem cell de-differentiation." in: Stem cells and development, (2012) (PubMed).

Hoppstädter, Diesel, Zarbock, Breinig, Monz, Koch, Meyerhans, Gortner, Lehr, Huwer, Kiemer: "Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages." in: Respiratory research, Vol. 11, pp. 124, (2010) (PubMed).

Kroeze, Jurgens, Doulabi, van Milligen, Scheper, Gibbs: "Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES." in: The Journal of investigative dermatology, Vol. 129, Issue 6, pp. 1569-81, (2009) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Chin-Yee, Keeney, Stewart, Belch, Bence-Buckler, Couban, Howson-Jan, Rubinger, Stewart, Sutherland, Paragamian, Bhatia, Foley: "Optimising parameters for peripheral blood leukapheresis after r-metHuG-CSF (filgrastim) and r-metHuSCF (ancestim) in patients with multiple myeloma: a temporal analysis of CD34(+) absolute counts and subsets." in: Bone marrow transplantation, Vol. 30, Issue 12, pp. 851-60, (2002) (PubMed).

Seiffert, Brossart, Cant, Cella, Colonna, Brugger, Kanz, Ullrich, Bühring: "Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells." in: Blood, Vol. 97, Issue 9, pp. 2741-9, (2001) (PubMed).

Ito, Inaba, Inaba, Toki, Sogo, Iguchi, Adachi, Yamaguchi, Amakawa, Valladeau, Saeland, Fukuhara, Ikehara: "A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 3, pp. 1409-19, (1999) (PubMed).

St Louis, Woodcock, Franzoso, Blair, Carlson, Murillo, Wells, Williams, Smoot, Kaushal, Grimes, Harlan, Chute, June, Siebenlist, Lee, Fransozo: "Evidence for distinct intracellular signaling pathways in CD34+ progenitor to dendritic cell differentiation from a human cell line model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3237-48, (1999) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Thy-1 cell surface antigen,CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.,Thy-1

Gene ID: 7070

UniProt: P04216

Pathways: Cell-Cell Junction Organization