8-OHDG antibody

Catalog No. ABIN781510

Product Details anti-8-OHDG Antibody

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This 8-OHDG antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Affinity Chromatography on Protein G

Clone: 15A3

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.65 mg/mL

Buffer: PBS containing 50 % Glycerol and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-8-OHDG antibody (ABIN781510)

Zawada, Mrak, Biedermann, Palmer, Gentleman, Aboud, Griffin et al.: "Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related ..." in: Acta neuropathologica communications, Vol. 3, pp. 9, (2015) (PubMed).

Angeloni, Malaguti, Rizzo, Barbalace, Fabbri, Hrelia: "Neuroprotective effect of sulforaphane against methylglyoxal cytotoxicity." in: Chemical research in toxicology, Vol. 28, Issue 6, pp. 1234-45, (2015) (PubMed).

Aboud, Mrak, Boop, Griffin: "Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype." in: Acta neuropathologica communications, Vol. 1, pp. 41, (2013) (PubMed).

Tomas, Wrzosek, Bouznad, Bouet, Mayeur, Noordine, Honvo-Houeto, Langella, Thomas, Cherbuy: "Primocolonization is associated with colonic epithelial maturation during conventionalization." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 2, pp. 645-55, (2013) (PubMed).

Brennan, Haukedal, Earle, Keddie, Harris: "Disruption of redox homeostasis leads to oxidative DNA damage in spermatocytes of Wolbachia-infected Drosophila simulans." in: Insect molecular biology, Vol. 21, Issue 5, pp. 510-20, (2012) (PubMed).

Kakinuma, Roy, Zhu, Wang, Kiyama: "Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling." in: The Journal of cell biology, Vol. 181, Issue 3, pp. 537-49, (2008) (PubMed).

Criollo, Maiuri, Tasdemir, Vitale, Fiebig, Andrews, Molgó, Díaz, Lavandero, Harper, Pierron, di Stefano, Rizzuto, Szabadkai, Kroemer: "Regulation of autophagy by the inositol trisphosphate receptor." in: Cell death and differentiation, Vol. 14, Issue 5, pp. 1029-39, (2007) (PubMed).

Kaneshiro, Ichihara, Takemitsu, Sakoda, Suzuki, Nakagawa, Hayashi, Inagami: "Increased expression of cyclooxygenase-2 in the renal cortex of human prorenin receptor gene-transgenic rats." in: Kidney international, Vol. 70, Issue 4, pp. 641-6, (2006) (PubMed).

Rendl, Lewis, Fuchs: "Molecular dissection of mesenchymal-epithelial interactions in the hair follicle." in: PLoS biology, Vol. 3, Issue 11, pp. e331, (2005) (PubMed).

Takebayashi, Amakawa, Tajima, Miyaji, Nakamura, Ito, Matsumoto, Miyazaki, Zen, Kishimoto, Fukuhara: "Blood dendritic cells are decreased in acute graft-versus-host disease." in: Bone marrow transplantation, Vol. 33, Issue 10, pp. 989-96, (2004) (PubMed).

Nagafuku, Kabayama, Oka, Kato, Tani-ichi, Shimada, Ohno-Iwashita, Yamasaki, Saito, Iwabuchi, Hamaoka, Inokuchi, Kosugi et al.: "Reduction of glycosphingolipid levels in lipid rafts affects the expression state and function of glycosylphosphatidylinositol-anchored proteins but does not impair signal transduction via the T cell ..." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51920-7, (2004) (PubMed).

Tajima, Amakawa, Ito, Miyaji, Takebayashi, Fukuhara: "Immunomodulatory effects of cyclosporin A on human peripheral blood dendritic cell subsets." in: Immunology, Vol. 108, Issue 3, pp. 321-8, (2003) (PubMed).

Cappelli, Volpe, Sanduzzi, Sacchi, Colizzi, Mariani et al.: "Human macrophage gamma interferon decreases gene expression but not replication of Mycobacterium tuberculosis: analysis of the host-pathogen reciprocal influence on transcription in a comparison of ..." in: Infection and immunity, Vol. 69, Issue 12, pp. 7262-70, (2001) (PubMed).

Sauermann, Stahl-Hennig, Stolte, Mühl, Krawczak, Spring, Fuchs, Kaup, Hunsmann, Sopper: "Homozygosity for a conserved Mhc class II DQ-DRB haplotype is associated with rapid disease progression in simian immunodeficiency virus-infected macaques: results from a prospective study." in: The Journal of infectious diseases, Vol. 182, Issue 3, pp. 716-24, (2000) (PubMed).

Weidmann, Maisner, Garten, Seufert, ter Meulen, Schneider-Schaulies: "Proteolytic cleavage of the fusion protein but not membrane fusion is required for measles virus-induced immunosuppression in vitro." in: Journal of virology, Vol. 74, Issue 4, pp. 1985-93, (2000) (PubMed).

Kawabata, Miller, Lehner, Fujihashi, Kubota, McGhee, Imaoka, Hiroi, Kiyono: "Induction of Th2 cytokine expression for p27-specific IgA B cell responses after targeted lymph node immunization with simian immunodeficiency virus antigens in rhesus macaques." in: The Journal of infectious diseases, Vol. 177, Issue 1, pp. 26-33, (1998) (PubMed).

Target Details for 8-OHDG

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Alternative Name: 8-Hydroxy-Guanosine / 8-OHG (8-OHDG Products)

Target Type: Chemical

Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).Synonyms: 8-Hydroxy-2'-deoxyguanosine, 8-Hydroxyguanine, 8-Hydroxyguanosine, 8-OFdG, 8-OHG, 8OG, 8OHG, 8OHdG