ESR2 antibody (AA 512-530)

Catalog No. ABIN781661

Product Details anti-ESR2 Antibody

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Binding Specificity: AA 512-530

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Monoclonal antibody 4ERβ-2D7 recognizes an epitope in the F region of the mouse and human Estrogen Receptor beta. The two different forms of the estrogen receptor, usually referred to as α and β, can form hormone-activated dimers, and, since the two forms are coexpressed in many cell types, the receptors may form ERα (αα) or ERβ (ββ) homodimers or ERαβ (αβ) heterodimers. Estrogen receptor alpha and beta show significant overall sequence homology, and both are composed of five domains (A-F domains listed from the N- to C-terminus). Antigen distribution: The antigen has been described in kidney, brain, bone, heart, lungs, intestinal mucosa, prostate, and endothelial cells. No cross-reactivity has been observedwith ER-alpha

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography

Immunogen: Peptide C512 to Q530 from murine ERbeta. Antigen: Estrogen Receptor beta. Epitope: Located in the F region.

Clone: ERbeta-2D7

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water

Concentration: 0.5 mg/mL

Buffer: Stock solution contains 0.5 mg/mL IgG, PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.01 % Kathon as a preservative.

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the lyophilized antibody at 2- 8 °C. Following reconstitution, store at -20 °C.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen Receptor beta (ESR2 Products)

Synonyms: ER-BETA antibody, ESR-BETA antibody, ESRB antibody, ESTRB antibody, Erb antibody, NR3A2 antibody, ER[b]1 antibody, zfER-beta1 antibody, zfER[b]1 antibody, erb2 antibody, CERB antibody, ERB antibody, erb antibody, esrb antibody, estrb antibody, nr3a2 antibody, ERbeta antibody, er-beta antibody, esr-beta antibody, ESR2 antibody, ERBETA antibody, er antibody, ER-beta antibody, ER[b] antibody, Estrb antibody, ER[b]2 antibody, ER[b]a antibody, zfER[b]2 antibody, zfER-beta2 antibody, Erb2 antibody, esr2 antibody, erbeta antibody, Esr2 antibody, ERBeta2 antibody, estrogen receptor 2 antibody, estrogen receptor 2b antibody, estrogen receptor beta 2 antibody, estrogen receptor 2 (beta) antibody, estrogen receptor 2a antibody, estrogen receptor beta antibody, ESR2 antibody, esr2b antibody, erb2 antibody, esr2 antibody, Esr2 antibody, esr2a antibody, erbeta antibody

Background: ER exists in two types: alpha and Beta. They are similar in structure. The ligand binding domain and the DNA binding domain are highly conserved in these proteins while the N-terminal transactivating domain is diverged considerably. Five isoforms of the hER beta gene, designated hER beta 1-5 have been identified. ERBeta binds to estrogen and activates genes through direct interaction with estrogen specific gene elements (ERE's). ER-Beta RNA has been detected in human thymus, spleen, ovary and testis and in rat ovary and prostrate. This tissue distribution overlaps but is not identical to that of ER - alphaRNA.Synonyms: ER-beta, ESR2, ESTRB, NR3A2, Nuclear receptor subfamily 3 group A member 2

Gene ID: 2100

NCBI Accession: NP_001035365

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling