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Lymphocyte Antigen 75 (LY75) antibody

Details for Product No. ABIN781804, Supplier: Log in to see
Antigen
  • CD205
  • CLEC13B
  • DEC-205
  • DEC205
  • GP200-MR6
  • im:7150926
  • Ly-75
  • LY-75
  • LY75
  • LYT75
  • MGC140308
  • RGD1565962
  • si:dkey-78p20.2
Reactivity
Human
155
78
20
19
6
4
Host
Mouse
120
62
50
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
32
32
12
9
7
6
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
152
62
51
39
35
32
21
11
9
8
7
7
4
3
2
2
Supplier
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Immunogen Recombinant Fc-tagged human CD205
Clone HD30
Isotype IgG1
Specificity This antibody recognizes CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Protein-A affinity chromatography
Purity > 95 % pure (by SDS-PAGE)
Alternative Name CD205 / DEC-205 (LY75 Antibody Abstract)
Background CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.Synonyms: C-type lectin domain family 13 member B, CLEC13B, DEC205, LY75, Lymphocyte antigen 75, gp200-MR6
Gene ID 100526664
NCBI Accession NP_001185688
UniProt O60449
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
Background publications Park, Rodriguez, Ueta, Lee, Pack, Matsuno, Steinman: "Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals." in: Journal of immunological methods, Vol. 377, Issue 1-2, pp. 15-22, 2012 (PubMed).

Gurer, Strowig, Brilot, Pack, Trumpfheller, Arrey, Park, Steinman, Münz: "Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses." in: Blood, Vol. 112, Issue 4, pp. 1231-9, 2008 (PubMed).

Mahnke, Guo, Lee, Sepulveda, Swain, Nussenzweig, Steinman: "The dendritic cell receptor for endocytosis, DEC-205, can recycle and enhance antigen presentation via major histocompatibility complex class II-positive lysosomal compartments." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 673-84, 2000 (PubMed).

Guo, Gong, Maric, Misulovin, Pack, Mahnke, Nussenzweig, Steinman: "A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells." in: Human immunology, Vol. 61, Issue 8, pp. 729-38, 2000 (PubMed).