Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody

Details for Product No. ABIN782921
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Antigen
Synonyms UBC9, T13J8.70, T13J8_70, UBIQUITIN-PROTEIN LIGASE, ubiquitin conjugating enzyme 9, C358B7.1, P18, 5830467E05Rik, F830028O17Rik, Ubce2i, Ubce9, UbcE2A, ubc9, ube2ia, zUbc9, ubce9
Epitope
N-Term
(33), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(101), (25), (20), (6), (3), (3), (2), (2), (2), (1), (1)
Host
Goat
(60), (24), (17), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (5), (5), (5)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(99), (58), (50), (10), (10), (9), (6), (6), (4), (4), (1), (1), (1)
Pubmed 3 references available
Quantity 20 μg
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1 Genename: UBE2I
Sequence SGIALSRLAQ ERK-C
Specificity This antibody detects UBC9 / UBE2I at N-term. It is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Cross-Reactivity (Details) Species reactivity (expected):Canien (Dog).
Species reactivity (tested):Human, Mouse, Rat.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name UBC9 / UBE2I (UBE2I Antibody Abstract)
Background Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
Gene ID 7329
NCBI Accession NP_003336
UniProt P63279
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (Image 2)
Background publications Subramaniam, Mealer, Sixt et al.: "Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20428-32, 2010 (PubMed).

Ihara, Stein, Schultz: "UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes." in: Biology of reproduction, Vol. 79, Issue 5, pp. 906-13, 2008 (PubMed).

Wang, Qiu, Seufert et al.: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, 1996 (PubMed).

Validation Images
Catalog No. ABIN782921
203.50 $
Plus shipping costs $45.00
Quantity
Price
20 μg
203.50 $

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