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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Internal Region) antibody

Details for Product No. ABIN783010, Supplier: Login to see
Antigen
  • cb1075
  • cb983
  • dmnt1
  • fb05h01
  • mta
  • wu:fb05h01
  • ADCADN
  • AIM
  • CXXC9
  • DNMT
  • HSN1E
  • MCMT
  • DDM2
  • DECREASED DNA METHYLATION 2
  • DMT01
  • DMT1
  • DNA METHYLTRANSFERASE
  • DNA METHYLTRANSFERASE 01
  • DNA METHYLTRANSFERASE 1
  • MET2
  • METHYLTRANSFERASE 2
  • METHYLTRANSFERASE I
  • METI
  • methyltransferase 1
  • Cxxc9
  • Dnmt
  • Dnmt1o
  • MTase
  • Met-1
  • Met1
  • MommeD2
  • m.MmuI
Epitope
Internal Region
59
42
35
28
22
22
20
19
13
13
13
12
9
9
9
7
7
7
6
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
387
109
40
40
36
4
4
4
3
2
1
1
1
1
1
1
Host
Goat
252
111
19
8
3
Clonality
Polyclonal
Conjugate
Un-conjugated
23
23
20
18
18
18
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
255
130
104
97
82
72
46
38
26
22
10
6
2
1
1
Supplier
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Immunogen Peptide with sequence from the internal egion of the protein sequence Genename: DNMT1
Sequence C-RFESPPKTQP TEDN
Specificity This antibody recognizes DNMT1.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine, Bovine, Porcine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt 1, Dnmt 2 and Dnmt 3. Dnmt 1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt 2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt 3a and Dnmt 3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F 1, and HDAC 1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity, as well as a growth regulatory pathway that is disrupted in nearly all cancer cells.Synonyms: AIM, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, HsaI, MCMT
Molecular Weight 183kDa (NP_001370.1).
Gene ID 1786
NCBI Accession NP_001124295
UniProt P26358
Research Area DNA/RNA
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Internal Region) antibody (ABIN783010) anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Internal Region) antibody
 image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Internal Region) antibody (ABIN783010) anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Internal Region) antibody (Image 2)
Product cited in: Dion, Lin, Hubert et al.: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." in: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, 2008 (PubMed).