Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 29-33)

Details for Product anti-CASP3 Antibody No. ABIN783311, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Alternatives
anti-Human Caspase 3 antibody for BioImaging
Epitope
AA 29-33
41
32
27
24
22
21
19
19
16
15
12
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11
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10
9
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5
5
5
5
5
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3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human
567
305
254
48
47
38
29
28
24
20
18
18
18
12
9
8
7
4
2
2
Host
Rabbit
458
162
19
4
2
Clonality
Polyclonal
Conjugate
This Caspase 3 antibody is un-conjugated
27
24
19
14
9
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
483
238
219
147
95
78
55
51
44
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
2
1
1
1
1
Options
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Immunogen Peptide sequence around aa.29~33 derived from Caspase 3
Sequence S-G-I-S-L
Specificity This antibody detects endogenous level of total Caspase 3 protein.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity-chromatography using epitope-specific peptide
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.Synonyms: Apopain, CASP-3, CASP3, CPP-32, CPP32, Cysteine protease CPP32, SCA-1, SCA1, SREBP cleavage activity 1, Yama protein
Molecular Weight 35/19/17 kDa
Gene ID 836
NCBI Accession NP_004337
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 29-33) (ABIN783311) Western blot analysis of extracts from 293, Hela and MDA cells using Caspase 3.
Background publications Gervais, Xu, Robertson, Vaillancourt, Zhu, Huang, LeBlanc, Smith, Rigby, Shearman, Clarke, Zheng, Van Der Ploeg, Ruffolo, Thornberry, Xanthoudakis, Zamboni, Roy, Nicholson: "Involvement of caspases in proteolytic cleavage of Alzheimer's amyloid-beta precursor protein and amyloidogenic A beta peptide formation." in: Cell, Vol. 97, Issue 3, pp. 395-406, 1999 (PubMed).

Nicholson, Ali, Thornberry, Vaillancourt, Ding, Gallant, Gareau, Griffin, Labelle, Lazebnik: "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis." in: Nature, Vol. 376, Issue 6535, pp. 37-43, 1995 (PubMed).