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Zinc Finger and BTB Domain Containing 4 (ZBTB4) (C-Term) antibody

Details for Product No. ABIN783355, Supplier: Log in to see
Antigen
  • KAISO-L1
  • ZNF903
  • 2310026P19Rik
  • 9230111I22Rik
  • RP23-422L16.16
  • mKIAA1538
Epitope
C-Term
15
10
10
7
2
2
Reactivity
Human, Mouse (Murine)
46
18
14
1
Host
Rabbit
46
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
34
21
11
6
3
1
1
1
Supplier
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Immunogen 18 amino acid peptide near the carboxy terminus of human ZBTB4
Specificity This antibody detects ZBTB4 (C-term). At least four isoforms of ZBTB4 are known to exist, this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Affinity chromatography purified via peptide column
Alternative Name ZBTB4 (ZBTB4 Antibody Abstract)
Background The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions, this degradation increases with DNA damage.Synonyms: KAISO-L1, KAISO-like zinc finger protein 1, KIAA1538, Zinc finger and BTB domain-containing protein 4
Gene ID 57659
NCBI Accession NP_001122305
UniProt Q9P1Z0
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Zinc Finger and BTB Domain Containing 4 (ZBTB4) (C-Term) antibody (ABIN783355) Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 μg/ml.
Background publications Yamada, Pérez-Torrado, Filion et al.: "The human protein kinase HIPK2 phosphorylates and downregulates the methyl-binding transcription factor ZBTB4." in: Oncogene, Vol. 28, Issue 27, pp. 2535-44, 2009 (PubMed).

Weber, Marquardt, Elzi et al.: "Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation." in: The EMBO journal, Vol. 27, Issue 11, pp. 1563-74, 2008 (PubMed).

Filion, Zhenilo, Salozhin et al.: "A family of human zinc finger proteins that bind methylated DNA and repress transcription." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 169-81, 2005 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).