ALKBH2 antibody (C-Term)

Catalog No. ABIN783675

Product Details anti-ALKBH2 Antibody

Target: ALKBH2 (AlkB, Alkylation Repair Homolog 2 (ALKBH2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography purified via peptide column

Immunogen: 15 amino acid peptide near the carboxy terminus of human ALKBH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ALKBH2

Target: ALKBH2 (AlkB, Alkylation Repair Homolog 2 (ALKBH2))

Alternative Name: ALKBH2 / ABH2 (ALKBH2 Products)

Synonyms: si:dkey-65b12.2 antibody, ABH2 antibody, 9530023G02 antibody, AU016977 antibody, Abh2 antibody, mABH2 antibody, RGD1306377 antibody, alkB homolog 2, alpha-ketoglutarate dependent dioxygenase antibody, alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase antibody, ALKBH2 antibody, alkbh2 antibody, Alkbh2 antibody

Background: The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA, ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.Synonyms: Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Oxy DC1

Gene ID: 121642

NCBI Accession: NP_001001655

UniProt: Q6NS38

Pathways: DNA Damage Repair