TFB2M antibody (Internal Region)

Catalog No. ABIN783976

Product Details anti-TFB2M Antibody

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TFB2M antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: KHCFGRRSAT VIDH

Specificity: Recognizes TFB2M.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_071761.1. Genename: TFB2M

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TFB2M

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Alternative Name: TFB2M (TFB2M Products)

Synonyms: CG3910 antibody, Dmel\\CG3910 antibody, TFB2 antibody, d-TF2BM antibody, d-mtTFB2 antibody, TFB2M antibody, sb:cb857 antibody, hkp1 antibody, Hkp1 antibody, mtTFB2 antibody, mitochondrial transcription factor B2 antibody, transcription factor B2, mitochondrial antibody, mtTFB2 antibody, TFB2M antibody, tfb2m antibody, Tfb2m antibody

Background: The transcription factors B1 (TFB1M, 607033) and B2 (TFB2M) are necessary for basal transcription of mammalian mitochondrial DNA. Human TFB1M and TFB2M are expressed ubiquitously and can each support promoter-specific mtDNA transcription in a pure recombinant in vitro system containing mitochondrial RNA polymerase and mitochondrial transcription factor A. TFB2M has been found to be at least 1 order of magnitude more active in promoting transcription than TFB1M. Both factors are highly homologous to bacterial rRNA dimethyltransferases, which suggests that an RNA-modifying enzyme has been recruited during evolution to function as a mitochondrial transcription factor.Synonyms: Dimethyladenosine transferase 2 mitochondrial, Hepatitis C virus NS5A-transactivated protein 5, Mitochondrial 12S rRNA dimethylase 2, Mitochondrial transcription factor B2, N'-adenosyl(rRNA) dimethyltransferase 2, NS5ATP5, S-adenosylmethionine-6-N', mtTFB2

Gene ID: 216

NCBI Accession: NP_071761