Albumin antibody (FITC)

Catalog No. ABIN784428

Product Details anti-Albumin Antibody (FITC)

Target: Albumin (ALB)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The defined antibody specificity is directed to Albumin as tested against Rat sera. In immunoelectrophoresis and Double Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified Albumin. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results: Mouse, Guinea Pig: ++ Horse: + Human, Swine, Monkey: ± Rabbit, Sheep, Goat, Canine, Chicken, Bovine: -Cross-reactivity

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Characteristics: Absorption / Emission: 492nm / 515nm

Purification: DEAE-column Chromatography

Immunogen: Highly purified Albumin isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives or foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin (ALB Products)

Synonyms: PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, ALB antibody, CSA antibody, Alb-1 antibody, Alb1 antibody, Albza antibody, LOC100136344 antibody, alb-a antibody, alb-b antibody, albumin antibody, serum albumin 1 antibody, albumin S homeolog antibody, ALB antibody, Alb antibody, LOC100136344 antibody, alb.S antibody

Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 24186

NCBI Accession: NP_599153

Pathways: Lipid Metabolism