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Antibodies
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
| Antigen |
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| Synonyms |
PKBB, PRKBB, PKBBETA, RAC-BETA, AKT2, cb945, prkbb, v-akt2, pkbbeta, rac-beta |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (99), Mouse (Murine) (42), Rat (Rattus) (39), Cow (Bovine) (3), Monkey (3), Chicken (2), Dog (Canine) (2), Guinea Pig (2), Hamster (2), Pig (Porcine) (2), Rabbit (2), Sheep (Ovine) (2), Xenopus laevis (2), Primate (1)
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Western Blotting (WB) (87), Immunofluorescence (IF) (35), ELISA (32), Flow Cytometry (FACS) (25), Immunohistochemistry (IHC) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Enzyme Immunoassay (EIA) (19), Immunoprecipitation (IP) (9), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN789374 |
| Quantity |
200ug (1 mg/ml in phosphate buffered salin...) (Variants) |
| Price |
420.50 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Akt2
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Immunogen
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Synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S)
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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Rabbit Anti-Akt2 (Phospho-Ser474) Polyclonal Antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.
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Application Notes
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WB: 1:500-1:1,000 IHC: 1:50-1:100
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Concentration
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1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
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Purification
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Rabbit Anti-Akt2 (Phospho-Ser474) Polyclonal Antibody is affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Storage
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Store the antibody at -20°C. It will remain stable for one year if stored properly.
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Restrictions
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For Research Use only
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Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)", type "Antibodies"
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Hosts
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Rabbit (73), Mouse (36), Chicken (1)
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Reactivities
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Human (99), Mouse (Murine) (42), Rat (Rattus) (39), Cow (Bovine) (3), Monkey (3), Chicken (2), Dog (Canine) (2), Guinea Pig (2), Hamster (2), Pig (Porcine) (2), Rabbit (2), Sheep (Ovine) (2), Xenopus laevis (2), Primate (1)
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Applications
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Western Blotting (WB) (87), Immunofluorescence (IF) (35), ELISA (32), Flow Cytometry (FACS) (25), Immunohistochemistry (IHC) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Enzyme Immunoassay (EIA) (19), Immunoprecipitation (IP) (9), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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PE (2), Agarose Beads (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer474 (10), C-Term (2), N-Term (2), Center (1), S474 (1), pS474 (1)
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