GLUD1 antibody

Catalog No. ABIN6758166

Product Details anti-GLUD1 Antibody

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified bovine liver glutamate dehydrogenase.

Purification: Ion exchange chromatography

Immunogen: Bovine liver glutamate dehydrogenase.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:6000 - 1:24000), IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GLUD1/Glutamate Dehydrogenase (GLUD1 Products)

Background: Name/Gene ID: GLUD1

Synonyms: GLUD1, GDH1, GDH 1, Glutamate dehydrogenase 1, Glutamate dehydrogenase 2, GDH, GLUD

Gene ID: 2746

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect