PDGFRA antibody (AA 1035-1053)

Catalog No. ABIN790499

Product Details anti-PDGFRA Antibody

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Binding Specificity: AA 1035-1053

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Monkey, Pig, Horse, Rabbit, Xenopus laevis, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C

Storage Comment: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-PDGFRA antibody (ABIN790499)

Nance, Shi, Hakim, Wasala, Yue, Pan, Zhang, Robinson, Duan, Yao, Yang, Chen, Wagner, Gersbach, Duan: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: PDGFRA / PDGFR Alpha (PDGFRA Products)

Synonyms: AI115593 antibody, CD140a antibody, Pdgfr-2 antibody, APDGFR antibody, PDGFACE antibody, CD140A antibody, PDGFR-2 antibody, PDGFR2 antibody, RHEPDGFRA antibody, PDGFR antibody, PDGFR-A antibody, platelet derived growth factor receptor alpha antibody, platelet derived growth factor receptor, alpha polypeptide antibody, platelet-derived growth factor receptor, alpha polypeptide L homeolog antibody, platelet-derived growth factor receptor, alpha polypeptide antibody, PDGFRA antibody, Pdgfra antibody, pdgfra.L antibody

Background: Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

Gene ID: 5156

UniProt: P16234

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling