PLA2G4F antibody (AA 781-795)

Catalog No. ABIN793126

Product Details anti-PLA2G4F Antibody

Target: PLA2G4F (Phospholipase A2, Group IVF)

Binding Specificity: AA 781-795

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PLA2G4F antibody is un-conjugated

Application: ELISA

Specificity: Human PLA2G4F / CPLA2-zeta.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%) Gorilla, Elephant (93%) Mouse, Bat (87%) Hamster, Bovine (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QTAEEKAFGDFVINR from an internal region (near C-terminus) of human PLA2G4F / CPLA2-zeta (NP_998765.3). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla, Elephant (93%), Mouse, Bat (87%), Hamster, Bovine (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 55-60kD and 35kD in Human Placenta lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kD according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PLA2G4F

Target: PLA2G4F (Phospholipase A2, Group IVF)

Alternative Name: PLA2G4F (PLA2G4F Products)

Synonyms: PLA2G4FZ antibody, 4732472I07Rik antibody, Gm647 antibody, Pla2zeta antibody, phospholipase A2 group IVF antibody, phospholipase A2, group IVF antibody, PLA2G4F antibody, Pla2g4f antibody

Background: Name/Gene ID: PLA2G4F

Synonyms: PLA2G4F, CPLA2-zeta, Phospholipase A2 group IVF, Phospholipase A2, group IVF, PLA2G4FZ, PLA2G4F/Z

Gene ID: 255189

NCBI Accession: NP_998765