IL-1 beta antibody (N-Term)
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- Target See all IL-1 beta (IL1B) Antibodies
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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Binding Specificity
- N-Term
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Reactivity
- Human, Dog, Primate
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL-1 beta antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
- Purification
- Purified IgG
- Immunogen
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Recombinant human IL1B / IL1 Beta.
Type of Immunogen: Recombinant protein - Top Product
- Discover our top product IL1B Primary Antibody
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- Application Notes
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Approved: ELISA (1:500 - 1:2000), IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)
Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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- Target
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- Alternative Name
- IL1B (IL1B Products)
- Synonyms
- IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody
- Background
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Name/Gene ID: IL1B
Family: IL-1
Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta - Gene ID
- 3553
- UniProt
- P01584
- Pathways
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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