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IL-1 beta antibody (N-Term)

IL1B Reactivity: Human, Dog, Primate WB, ELISA, IHC, IP, IHC (p), RIA, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN799612
  • Target See all IL-1 beta (IL1B) Antibodies
    IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
    Binding Specificity
    • 17
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    • 2
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    • 1
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    N-Term
    Reactivity
    • 123
    • 87
    • 43
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    • 14
    • 8
    • 8
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    • 5
    • 3
    • 3
    • 1
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    Human, Dog, Primate
    Host
    • 175
    • 64
    • 6
    • 6
    • 4
    • 2
    • 1
    Rabbit
    Clonality
    • 181
    • 75
    • 1
    Polyclonal
    Conjugate
    • 136
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    • 2
    This IL-1 beta antibody is un-conjugated
    Application
    • 184
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    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
    Purification
    Purified IgG
    Immunogen
    Recombinant human IL1B / IL1 Beta.

    Type of Immunogen: Recombinant protein
    Top Product
    Discover our top product IL1B Primary Antibody
  • Application Notes
    Approved: ELISA (1:500 - 1:2000), IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)

    Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C. Avoid freeze-thaw cycles.
  • Target
    IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
    Alternative Name
    IL1B (IL1B Products)
    Synonyms
    IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody
    Background
    Name/Gene ID: IL1B
    Family: IL-1

    Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta
    Gene ID
    3553
    UniProt
    P01584
    Pathways
    NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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