IL1RN antibody
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- Target See all IL1RN Antibodies
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL1RN antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Radioimmunoassay (RIA)
- Specificity
- Recombinant human sIL-1Ra produced in E. coli.
- Purification
- Purified IgG
- Immunogen
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Recombinant human IL1RN / IL-1RA.
Type of Immunogen: Recombinant protein - Top Product
- Discover our top product IL1RN Primary Antibody
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- Application Notes
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Approved: ELISA (1:500 - 1:1000), Flo, IHC, RIA, WB
Usage: This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1 Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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- Target
- IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
- Alternative Name
- IL1RN (IL1RN Products)
- Synonyms
- DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MVCD4 antibody, IRAP1 antibody, IL1RN antibody, IL-1RA antibody, F630041P17Rik antibody, interleukin 1 receptor antagonist antibody, interleukin 1 receptor antagonist-like antibody, IL1RN antibody, Il1rn antibody, IL1RNL antibody
- Background
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Name/Gene ID: IL1RN
Family: IL-1
Synonyms: IL1RN, Anakinra, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3 - Gene ID
- 3557
- UniProt
- P18510
- Pathways
- NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
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